dba fluorescein  (Vector Laboratories)

Journal: Journal of Translational Autoimmunity

Article Title: T cell proliferative response to a homocitrullinated peptide correlates with joint pathology in collagen induced arthritis

doi: 10.1016/j.jtauto.2025.100345

Figure Lengend Snippet: Phenotypic Characterization of Collagen Induced Arthritis in DBA/1J Mice. DBA/1J mice were immunized with bovine type II collagen (N = 11–55; arthritic: n = 6–18; non-arthritic: n = 5–37) or PBS (N = 4–14). A) Representative photographs and E) 3D-rendered micro-CT radiographs of arthritic (red arrow = bone erosion), non-arthritic, and PBS-injected mouse hind limbs. Changes in B) clinical arthritis score, C) anti-bovine type II collagen (CII) IgG and D) anti-mouse CII IgG over time. Dashed line represents the antibody positivity cut-off, indicating the limit of detection. F) Hind limb bone mineral density and G) bone mineral density of bCII-immunized fore and hind limbs over time. H) Pain-like behaviour at day 47 post primary immunization. Graphs show the median [IQR], with each symbol in F-H) representing an individual mouse. For B-D), statistical analysis was performed using a mixed effects model with the Geisser-Greenhouse correction and Tukey's multiple comparisons test, with resulting p-values shown in the graphs. Kruskal-Wallis with Dunn's multiple comparisons test was used for F) and H), and the resulting p-values were p = 0.0008 and p = 0.0003 respectively, with p-values for pairwise comparisons indicated on the graphs. For G), a Spearman correlation coefficient (r) was calculated and p < 0.05 was considered significant.

Article Snippet: Male DBA/1J mice (000670; Jackson Laboratories; USA) were co-housed at the conventional facility at Western University according to the Canadian Council on Animal Care guidelines.

Techniques: Micro-CT, Injection

Journal: Journal of Translational Autoimmunity

Article Title: T cell proliferative response to a homocitrullinated peptide correlates with joint pathology in collagen induced arthritis

doi: 10.1016/j.jtauto.2025.100345

Figure Lengend Snippet: Collagen Induced Arthritis Histopathology in DBA/1J Metatarsophalangeal Joints. Representative A) hematoxylin + eosin and B) toluidine blue micrographs of joints from DBA/1J mice immunized with bovine type II collagen (arthritic: N = 9; non-arthritic: N = 11), or PBS (N = 14). The first image in each row was obtained at 140× magnification (scale bar = 300 μm). Adjacent images show enlargement of the outlined regions, obtained at 400× magnification (scale bar = 100 μm). Histopathological features: pannus (yellow arrowheads) with bone marrow penetration (green asterisk), joint debris (green arrows), synovitis (black asterisk), proteoglycan loss (yellow oval), and cartilage erosion (red arrowheads). C) Four histopathology parameters, synovial inflammation, bone erosion, proteoglycan loss, and cartilage erosion, were each scored out of 3. Graphs show the median [IQR], with each symbol representing an individual mouse. Statistical analysis was performed using Kruskal-Wallis with Dunn's multiple comparisons test, and the resulting p-values were p = 0.0013, p = 0.0003, p = 0.0016, and p = 0.0003 respectively, with p-values for pairwise comparisons indicated on the graphs.

Article Snippet: Male DBA/1J mice (000670; Jackson Laboratories; USA) were co-housed at the conventional facility at Western University according to the Canadian Council on Animal Care guidelines.

Techniques: Histopathology

Journal: Journal of Translational Autoimmunity

Article Title: T cell proliferative response to a homocitrullinated peptide correlates with joint pathology in collagen induced arthritis

doi: 10.1016/j.jtauto.2025.100345

Figure Lengend Snippet: Citrullinated and Homocitrullinated Proteins/Peptides are Present in DBA/1J Metatarsophalangeal Joints. A) Representative hematoxylin + eosin (H&E) at 80× magnification and immunofluorescent micrographs at 20× magnification of joints from DBA/1J mice immunized with bovine type II collagen (arthritic: N = 9; non-arthritic: N = 10) or PBS (N = 9). Scale bars represent 500 μm. Max projections of Z-stacks show nuclei (DAPI; blue), citrullinated proteins/peptides (CitP; green), and homocitrullinated proteins/peptides (HomoCitP; red). Corrected total fluorescence intensity for B) CitP and C) HomoCitP in three joint structures: bone marrow, synovium, and cartilage. Graphs show the median [IQR], with each symbol representing an individual mouse. Statistical analysis was performed using Kruskal-Wallis with Dunn's multiple comparisons test, and the resulting p-values were p = 0.0046, p = 0.0084, p = 0.4076, p = 0.0143, p = 0.0136, and p = 0.1733 respectively, with p-values for pairwise comparisons indicated on the graphs. ns: not significant.

Article Snippet: Male DBA/1J mice (000670; Jackson Laboratories; USA) were co-housed at the conventional facility at Western University according to the Canadian Council on Animal Care guidelines.

Techniques: Fluorescence

Journal: Journal of Translational Autoimmunity

Article Title: T cell proliferative response to a homocitrullinated peptide correlates with joint pathology in collagen induced arthritis

doi: 10.1016/j.jtauto.2025.100345

Figure Lengend Snippet: Splenic T Cell Proliferation Following Stimulation with Type II Collagen Protein. Splenocytes isolated at day 49 post primary immunization from DBA/1J mice immunized with bovine type II collagen (bCII) (arthritic: N = 8; non-arthritic: N = 7) or PBS (N = 10) were cultured in media alone or 80 μg/mL bCII protein. Following 72 h incubation, A) CD4 + and B) CD8 + T cell proliferation following stimulation with bCII was measured by flow cytometry and is shown as a mean (±SD) stimulation index, with data points representing individual mice. Stimulation indices greater than 1.6 (dashed line) were considered a positive response. Statistical analysis was performed using one-way ANOVA with Tukey's multiple comparisons test, and the resulting p-values were p = 0.0054 and p = 0.0987 respectively, with p-values for pairwise comparisons indicated on the graphs. ns: not significant.

Article Snippet: Male DBA/1J mice (000670; Jackson Laboratories; USA) were co-housed at the conventional facility at Western University according to the Canadian Council on Animal Care guidelines.

Techniques: Isolation, Cell Culture, Incubation, Flow Cytometry

Journal: Journal of Translational Autoimmunity

Article Title: T cell proliferative response to a homocitrullinated peptide correlates with joint pathology in collagen induced arthritis

doi: 10.1016/j.jtauto.2025.100345

Figure Lengend Snippet: Splenic T Cell Proliferation Following Stimulation with Citrullinated and Homocitrullinated Peptides . Splenocytes isolated at day 49 post primary immunization from DBA/1J mice immunized with bovine type II collagen (arthritic: N = 8; non-arthritic N = 7), or PBS (N = 10) were cultured in media alone, 100 μg/mL citrullinated peptide (CitP), or 100 μg/mL homocitrullinated peptide (HomoCitP). Following 72 h incubation, CD4 + and CD8 + T cell proliferation following stimulation with A-B) CitP and C-D) HomoCitP was measured by flow cytometry and is shown as a mean (±SD) stimulation index, with data points representing individual mice. Stimulation indices greater than 1.6 (dashed line) were considered a positive response. Statistical analysis was performed using one-way ANOVA with Tukey's multiple comparisons test, and the resulting p-values were p = 0.5857, p = 0.3012, p = 0.0036, and p = 0.0024 respectively, with p-values for pairwise comparisons indicated on the graphs. ns: not significant.

Article Snippet: Male DBA/1J mice (000670; Jackson Laboratories; USA) were co-housed at the conventional facility at Western University according to the Canadian Council on Animal Care guidelines.

Techniques: Isolation, Cell Culture, Incubation, Flow Cytometry

Journal: Journal of Translational Autoimmunity

Article Title: T cell proliferative response to a homocitrullinated peptide correlates with joint pathology in collagen induced arthritis

doi: 10.1016/j.jtauto.2025.100345

Figure Lengend Snippet: RA-Specific Serum IgG Antibodies in DBA/1J Mice. Sera were collected biweekly from DBA/1J mice immunized with bovine type II collagen (bCII) (arthritic: N = 9–18; non-arthritic: N = 17–37), or PBS (N = 14) and tested via indirect ELISA for A) anti-CitP, and B) anti-HomoCitP IgG antibodies. Graphs show the median [IQR] for each group, with a cut-off indicating the limit of detection (dashed line). Statistical analysis was performed using a mixed effects model with the Geisser-Greenhouse correction and Tukey's multiple comparisons test. The resulting p-values are indicated on the graphs.

Article Snippet: Male DBA/1J mice (000670; Jackson Laboratories; USA) were co-housed at the conventional facility at Western University according to the Canadian Council on Animal Care guidelines.

Techniques: Indirect ELISA

Journal: iScience

Article Title: Compensatory responses to glaucoma pathology in the dorsolateral geniculate nucleus

doi: 10.1016/j.isci.2026.115176

Figure Lengend Snippet: Intraocular pressure and glaucoma pathology in DBA/2J mice (A) Peak IOP measurements from individual eyes in the current study show significantly increased intraocular pressure in D2 mice compared to D2-controls [∗∗∗∗p << 0.0001 with Mann Whitney test; n = 71 mice, 142 eyes (D2-control) and n = 66 mice, 132 eyes (D2)]. Individual data points represent the measurement from an individual eye. (B) Cross section images of optic nerves from 12-month-old D2 and D2-control animals. Scale bars represent 200 μm. (C) Glial scarring as a percentage of cross-sectional optic nerve area was quantified in D2 and D2-controls (∗∗ p = 0.0071 with t test; N = 12 nerves from seven mice [D2-control] and N = 9 nerves from six mice [D2]). Bars and error show mean ± SEM. (D) Individual (gray and light red) and average (black, dark red) pattern electroretinogram (pERG) waveforms from D2-control (black) and D2 (red) mice. n = 16 eyes from eight mice (D2-control) and n = 14 eyes from seven mice. (E) P1 and N2 pERG amplitudes measured from pre-stimulus baseline in D2-control (black) and D2 (red) mice. P1 and N2 amplitude measurements were significantly decreased in D2 mice compared to D2-controls (∗∗∗∗ p = 0.000056 [P1] and ∗∗∗ p = 0.00033 [N2]; n = 16 eyes from eight mice [D2-control] and n = 14 eyes from seven mice [D2]). Bars and error show mean ± SEM. (F) Linear regression of P1 pERG amplitude with maximum IOP, showing a significant correlation. Best fit line and 95% confidence intervals are shown. (G) Linear regression of P1 pERG amplitude with glial scarring, showing a weak but significant correlation. Best fit line and 95% confidence intervals are shown.

Article Snippet: DBA/2J mice (D2, The Jackson Laboratory #000671, RRID:IMSR_JAX:000671) and DBA/2J- Gpnmb1 + mice (D2-control, The Jackson Laboratory #007048, RRID:IMSR_JAX:007048) were used for this study at 11-15 months of age, although most mice in this study were 11-13 months of age.

Techniques: MANN-WHITNEY, Control

Journal: iScience

Article Title: Compensatory responses to glaucoma pathology in the dorsolateral geniculate nucleus

doi: 10.1016/j.isci.2026.115176

Figure Lengend Snippet: Pharmacology of inhibition indicates decreased activation of extrasynaptic GABA A receptors in DBA/2J TC neurons (A) Example peak-normalized IPSCs from TC neurons from D2 and D2-control mice evoked by stimulation with an aCSF-filled patch pipette positioned approximately 25 microns from the recorded TC neuron in the presence of CNQX (20 μM) and D-AP5 (50 μM). Traces show before (gray) and after (black) bath application of 10 μM DS2. (B) Relative effect of DS2 application on peak response of the IPSC (n.s., p = 0.16 via nested t test) and area (∗∗∗ p = 0.0010 via nested t test) of D2 (red) and D2-control (black) mice ( n = 8 cells, four mice [D2-control] and n = 16 cells, six mice [D2]). Bar graphs and error bars show mean ± SEM. (C) Example peak-normalized traces of IPSCs from D2 and D2-control mice before (gray) and after (black) application of SNAP5114 (60 μM). IPSCs were evoked as in A. (D) Relative effect of SNAP5114 application on peak response (n.s., p = 0.70 via nested t test) and area (n.s., p = 0.91 via nested t test) of D2 (red) and D2-control (black) mice ( n = 16 cells, six mice [D2] and n = 14 cells, five mice [D2-control]). Bar graphs and error bars show mean ± SEM.

Article Snippet: DBA/2J mice (D2, The Jackson Laboratory #000671, RRID:IMSR_JAX:000671) and DBA/2J- Gpnmb1 + mice (D2-control, The Jackson Laboratory #007048, RRID:IMSR_JAX:007048) were used for this study at 11-15 months of age, although most mice in this study were 11-13 months of age.

Techniques: Inhibition, Activation Assay, Control, Transferring

Journal: iScience

Article Title: Compensatory responses to glaucoma pathology in the dorsolateral geniculate nucleus

doi: 10.1016/j.isci.2026.115176

Figure Lengend Snippet: Intraocular pressure and glaucoma pathology in DBA/2J mice (A) Peak IOP measurements from individual eyes in the current study show significantly increased intraocular pressure in D2 mice compared to D2-controls [∗∗∗∗p << 0.0001 with Mann Whitney test; n = 71 mice, 142 eyes (D2-control) and n = 66 mice, 132 eyes (D2)]. Individual data points represent the measurement from an individual eye. (B) Cross section images of optic nerves from 12-month-old D2 and D2-control animals. Scale bars represent 200 μm. (C) Glial scarring as a percentage of cross-sectional optic nerve area was quantified in D2 and D2-controls (∗∗ p = 0.0071 with t test; N = 12 nerves from seven mice [D2-control] and N = 9 nerves from six mice [D2]). Bars and error show mean ± SEM. (D) Individual (gray and light red) and average (black, dark red) pattern electroretinogram (pERG) waveforms from D2-control (black) and D2 (red) mice. n = 16 eyes from eight mice (D2-control) and n = 14 eyes from seven mice. (E) P1 and N2 pERG amplitudes measured from pre-stimulus baseline in D2-control (black) and D2 (red) mice. P1 and N2 amplitude measurements were significantly decreased in D2 mice compared to D2-controls (∗∗∗∗ p = 0.000056 [P1] and ∗∗∗ p = 0.00033 [N2]; n = 16 eyes from eight mice [D2-control] and n = 14 eyes from seven mice [D2]). Bars and error show mean ± SEM. (F) Linear regression of P1 pERG amplitude with maximum IOP, showing a significant correlation. Best fit line and 95% confidence intervals are shown. (G) Linear regression of P1 pERG amplitude with glial scarring, showing a weak but significant correlation. Best fit line and 95% confidence intervals are shown.

Article Snippet: DBA/2J mice (D2, The Jackson Laboratory #000671, RRID:IMSR_JAX:000671) and DBA/2J- Gpnmb1 + mice (D2-control, The Jackson Laboratory #007048, RRID:IMSR_JAX:007048) were used for this study at 11-15 months of age, although most mice in this study were 11-13 months of age.

Techniques: MANN-WHITNEY, Control

Journal: iScience

Article Title: Compensatory responses to glaucoma pathology in the dorsolateral geniculate nucleus

doi: 10.1016/j.isci.2026.115176

Figure Lengend Snippet: Pharmacology of inhibition indicates decreased activation of extrasynaptic GABA A receptors in DBA/2J TC neurons (A) Example peak-normalized IPSCs from TC neurons from D2 and D2-control mice evoked by stimulation with an aCSF-filled patch pipette positioned approximately 25 microns from the recorded TC neuron in the presence of CNQX (20 μM) and D-AP5 (50 μM). Traces show before (gray) and after (black) bath application of 10 μM DS2. (B) Relative effect of DS2 application on peak response of the IPSC (n.s., p = 0.16 via nested t test) and area (∗∗∗ p = 0.0010 via nested t test) of D2 (red) and D2-control (black) mice ( n = 8 cells, four mice [D2-control] and n = 16 cells, six mice [D2]). Bar graphs and error bars show mean ± SEM. (C) Example peak-normalized traces of IPSCs from D2 and D2-control mice before (gray) and after (black) application of SNAP5114 (60 μM). IPSCs were evoked as in A. (D) Relative effect of SNAP5114 application on peak response (n.s., p = 0.70 via nested t test) and area (n.s., p = 0.91 via nested t test) of D2 (red) and D2-control (black) mice ( n = 16 cells, six mice [D2] and n = 14 cells, five mice [D2-control]). Bar graphs and error bars show mean ± SEM.

Article Snippet: DBA/2J mice (D2, The Jackson Laboratory #000671, RRID:IMSR_JAX:000671) and DBA/2J- Gpnmb1 + mice (D2-control, The Jackson Laboratory #007048, RRID:IMSR_JAX:007048) were used for this study at 11-15 months of age, although most mice in this study were 11-13 months of age.

Techniques: Inhibition, Activation Assay, Control, Transferring

Journal: iScience

Article Title: Compensatory responses to glaucoma pathology in the dorsolateral geniculate nucleus

doi: 10.1016/j.isci.2026.115176

Figure Lengend Snippet: Intraocular pressure and glaucoma pathology in DBA/2J mice (A) Peak IOP measurements from individual eyes in the current study show significantly increased intraocular pressure in D2 mice compared to D2-controls [∗∗∗∗p << 0.0001 with Mann Whitney test; n = 71 mice, 142 eyes (D2-control) and n = 66 mice, 132 eyes (D2)]. Individual data points represent the measurement from an individual eye. (B) Cross section images of optic nerves from 12-month-old D2 and D2-control animals. Scale bars represent 200 μm. (C) Glial scarring as a percentage of cross-sectional optic nerve area was quantified in D2 and D2-controls (∗∗ p = 0.0071 with t test; N = 12 nerves from seven mice [D2-control] and N = 9 nerves from six mice [D2]). Bars and error show mean ± SEM. (D) Individual (gray and light red) and average (black, dark red) pattern electroretinogram (pERG) waveforms from D2-control (black) and D2 (red) mice. n = 16 eyes from eight mice (D2-control) and n = 14 eyes from seven mice. (E) P1 and N2 pERG amplitudes measured from pre-stimulus baseline in D2-control (black) and D2 (red) mice. P1 and N2 amplitude measurements were significantly decreased in D2 mice compared to D2-controls (∗∗∗∗ p = 0.000056 [P1] and ∗∗∗ p = 0.00033 [N2]; n = 16 eyes from eight mice [D2-control] and n = 14 eyes from seven mice [D2]). Bars and error show mean ± SEM. (F) Linear regression of P1 pERG amplitude with maximum IOP, showing a significant correlation. Best fit line and 95% confidence intervals are shown. (G) Linear regression of P1 pERG amplitude with glial scarring, showing a weak but significant correlation. Best fit line and 95% confidence intervals are shown.

Article Snippet: DBA/2J mouse (“D2”) , The Jackson Laboratory , Strain: 000671; RRID:IMSR_JAX:000671.

Techniques: MANN-WHITNEY, Control

Journal: iScience

Article Title: Compensatory responses to glaucoma pathology in the dorsolateral geniculate nucleus

doi: 10.1016/j.isci.2026.115176

Figure Lengend Snippet: Pharmacology of inhibition indicates decreased activation of extrasynaptic GABA A receptors in DBA/2J TC neurons (A) Example peak-normalized IPSCs from TC neurons from D2 and D2-control mice evoked by stimulation with an aCSF-filled patch pipette positioned approximately 25 microns from the recorded TC neuron in the presence of CNQX (20 μM) and D-AP5 (50 μM). Traces show before (gray) and after (black) bath application of 10 μM DS2. (B) Relative effect of DS2 application on peak response of the IPSC (n.s., p = 0.16 via nested t test) and area (∗∗∗ p = 0.0010 via nested t test) of D2 (red) and D2-control (black) mice ( n = 8 cells, four mice [D2-control] and n = 16 cells, six mice [D2]). Bar graphs and error bars show mean ± SEM. (C) Example peak-normalized traces of IPSCs from D2 and D2-control mice before (gray) and after (black) application of SNAP5114 (60 μM). IPSCs were evoked as in A. (D) Relative effect of SNAP5114 application on peak response (n.s., p = 0.70 via nested t test) and area (n.s., p = 0.91 via nested t test) of D2 (red) and D2-control (black) mice ( n = 16 cells, six mice [D2] and n = 14 cells, five mice [D2-control]). Bar graphs and error bars show mean ± SEM.

Article Snippet: DBA/2J mouse (“D2”) , The Jackson Laboratory , Strain: 000671; RRID:IMSR_JAX:000671.

Techniques: Inhibition, Activation Assay, Control, Transferring